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Teacher and Teaching Effects on Students’ Attitudes and Behaviors
This semester, three master students at CIGENE have completed their thesis with excellent results. We congratulate Dominiki ManousiHanna Magdalena Sahlström and Jostein Arntsen with their great accomplishments and wish you all the best in your future work.
Genotype imputation is an in silico inference method that increases the genotyping density of large populations. Using long-read negative effect not completing masters thesis, an improved genome assembly for salmon has recently been released. We tested the power of the new reference genome in terms of genotype imputation accuracy and compared results against the previous assembly version.
We report large genomic rearrangements and increase in chromosome length in the new genome assembly, followed by drastic improvement on imputation accuracy for regions of sequence similarity. Testing the applicability of currently available genotyping panels, we show good overall imputation performance but also heterogeneity in genotyping coverage, locally decreasing imputation accuracy performance.
Figure : Local imputation accuracy comparison between the new red line and old blue line genome assembly in selected chromosomes. Local performance was assessed as the mean r2 accuracy in groups of markers points in line. The density in points across each line indicates the genotyping density for the respective regions. Supervisors: Simen Rød Sandve negative effect not completing masters thesis Alex Kojo Datsomor.
Specific TEs have been linked to genes with increased expression in liver cells in Atlantic salmon. We functionally tested these TEs and determined if they impact gene expression. Our results clearly show that the TEs do not lead to increased gene expression, and may even act as repressive elements. Figure: The graph included shows a negative control, two positive control and the TEs presenting with lower expression than the negative control.
Sadly I do not have any pictures of myself in the lab, negative effect not completing masters thesis. Whole genome CRISPR screens have recently emerged as powerful tools for discovery of key genes that determine susceptibility to infectious diseases. Such screens can be greatly facilitated by creating cell lines that stably produce defined levels of Cas9 nuclease.
During the course of the project we used IPEC J2 cell line, derived from the pig intestine, to create a series of clones that produce different levels of Cas9. We have subsequently tested theses lines for their ability to support targeted gene editing.
Figure : Western blot of IPEC J2 clones lysates that illustrating different levels of endogenous Cas9 protein. by Lise Fjellsbø Posted on June 28, News. Domniki Manousi Assessing the effects of genotype imputation in the new Atlantic salmon Salmo salar genome assembly Supervisors: Simen Rød Sandve and Tim Martin Knutsen. Share this: Click to share on Twitter Opens in new window Click to share on Facebook Opens in new window.
Like this: Like Loading New symposium series arranged by CIGENE. Young research talent: Marie Saitou from CIGENE is assigned prestigious project funding. Leave a Reply Cancel reply. Loading Comments Email Required Name Required Website.
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